Dot-blot hybridization and RT-PCR detection of extra small virus (XSV) associated with white tail disease of prawn Macrobrachium rosenbergii
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چکیده
منابع مشابه
Monoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus.
The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S-transferase (GST)-fusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal antibodies (MAbs). Using dot blot, Western blot,...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2004
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao058083